Cryopreservation of in vitro-grown shoot tips of chrysanthemum by encapsulation-dehydration
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چکیده
منابع مشابه
Cryopreservation of in vitro-grown shoot tips of cassava by encapsulation-vitrification method.
Shoot tips of cassava (Manihot esculenta Crantz) in vitro plantlets were successfully cryopreserved using the encapsulation-vitrification technique. Nodal cuttings of 5 mm length with one leaf were cultured on modified MS medium in Petri dishes (90 mm x 20 mm) for about 28 days. Excised shoot tips were precultured on sucrose enriched (0.3 M) medium for 16 h, encapsulated and osmoprotected with ...
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In vitro shoot tips of Capparis spiunosa were cryopreserved using the vitrification, encapsulation/ dehydration and encapsulation vitrification techniques. In the vitrification procedure, a maximum of 70% regrwoth was obtained when shoot tips were treated with the Plant vitrification solution (PVS2) for 60 min at 0°C and plunged directly into liquid nitrogen. In encapsulation dehydration, shoot...
متن کاملCryopreservation of shoot tips of blackberry and raspberry by encapsulation-dehydration and vitrification.
Encapsulation-dehydration and PVS2-vitrification cryopreservation protocols were evaluated for the long-term conservation of a diverse group of Rubus germplasm. Cold acclimation for a 4-week period prior to cryopreservation was necessary for regrowth of shoot apices from blackberry and raspberry genotypes. For the encapsulation-dehydration protocol, encapsulated apices were pretreated in 0.75 M...
متن کاملCryopreservation of in vitro-grown shoot-tips of tropical taro (Colocasia esculenta var. esculenta) by vitrification.
In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. esculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were pre...
متن کاملCryopreservation of in vitro-grown shoot tips of papaya (Carica papaya L.) by vitrification
Shoot tips from 4to 6-week-old in vitro grown plantlets of six different cultivars of papaya were cryopreserved by vitrification. Shoot tips were treated in a 2 ml cryo-tube filled with a solution of 2 M glycerol and 0.4 M sucrose at 25°C for 20 min, then dehydrated with 1 ml pre-cooled vitrification solution at 4°C for 60 min. Treated samples were kept in liquid nitrogen for at least one week....
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ژورنال
عنوان ژورنال: Folia Horticulturae
سال: 2013
ISSN: 2083-5965
DOI: 10.2478/fhort-2013-0015